Среда DMEM/F-12 Culture Basic Media, 500 мл

Product Description: DMEM/F-12 is a 1:1 mixture of DMEM and Ham’s F-12, and it combines DMEM’s high concentrations of glucose, amino acids, and vitamins with F-12’s wide variety of components. DMEM/F-12 is widely used for supporting the growth of many different mammalian cells, including MDCK, glial cells, fibroblasts, human endothelia cells, and rat fibroblasts. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO₂ environment to maintain physiological pH. All raw material components used for Servicebio cell culture media products are screened through using strict quality control testing. Servicebio cell culture media are manufactured at a cGMP-compliant facility using water for injection, filtered through final 0.1 μm sterile filtration and aseptic filled in a Hundred-stage FFU. The filter integrity tests are performed before and after filtration. Each lot of cell culture media are subjected to sterility test, pH, osmolality, endotoxin and cell culture test. The major character of this DMEM is as follows: l With 17.5 mM Glucose, 2.5 mM L-glutamine, 0.5 mM sodium pyruvate, phenol red. l Without HEPES. The complete formulation is available. Storage: Storage conditions: 2℃ to 8℃, protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture